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Other
Part:BBa_K3385075:Design
Designed by: Daniel Bavnh�j Group: iGEM20_DTU-Denmark (2020-10-20)
PacI/Nt.BbvCI cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It's needed for integration of the CRISPR constructs using the restriction enzymes PacI and Nt.BbvCI.
Source
The part was obtained from pFC330[1].
References
[1] A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi. Nodvig CS, Nielsen JB, Kogle ME, Mortensen UH. PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015. PONE-D-15-11561 [pii] PubMed 26177455